Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.0) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
ProteinSimple Sample Buffer, 440 µL, Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems.
Wes, Sally Sue, Peggy Sue 10X Sample Buffer (440 uL).
Gold Biotechnology Inc HEPES, Sodium Salt 500 g
HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 500 g
Linear Formula: NH2C(CH2OH)3, Molecular Weight: 121.14, Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration). Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.
Boston Bioproducts Inc Paraformaldehyde (4%, PBS) - 250ml
Paraformaldehyde solution is a neutral fixative solution used to fix tissues and cells in immunohistochemistry and immunocytochemistry protocols. Stabilized in a phosphate buffer for delivery, this reagent actively cross-links proteins and DNA, immobilizing cellular components and maintaining the integrity of cell morphology. This reagent supports the accurate visualization of cells and tissues under a microscope, ensuring that researchers can observe fine details without degradation.
Boston Bioproducts Inc MES Buffer (1 M, pH 6.5) - 500ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Boston Bioproducts Inc Tris-Glycine-SDS Running Buffer 10X, 4 L, 8.50 ± 0.20 pH, 250 mM Tris, 1.92 M Glycine, 1% SDS, Anode and Cathode buffer, SDS-PAGE, Western blotting
This Tris-Glycine SDS Running Buffer (10X) is specifically formulated for use in protein electrophoresis applications, such as SDS-PAGE and Western blotting. It plays a vital role in the separation and resolution of denatured proteins. Upon dilution to the recommended working concentration, the buffer ensures consistent results with well-resolved protein bands. Furthermore, this buffer can function effectively as both the anode and cathode buffer, maintaining the pH essential for optimum electrophoresis performance.
- Volume: 4 L
- Concentration: 10X
- pH: 8.50 ± 0.20
- Application: SDS-PAGE, Western blotting
- Function: Separation of denatured proteins
- Recommended dilution: 10-fold with deionized water for 1X use
- Storage conditions: Room temperature
- Filtered through: 1-micron filter
- Quality control testing: Appearance, pH
General Laboratory Products Phosphate Buffered Water, 9mL 100/pk
Phosphate Buffered Water - 9ml, 16x125mm polycarbonate tube, 100 per case. For use as a diluent in microbial limit testing of food and water.
Rockland Immunochemicals 50X Tris-Acetate-EDTA (TAE) (2.0 M Tris-Acetate 0.05 M EDTA pH 8.3) - MB-020
Tris-Acetate-EDTA (TAE) is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. TAE suitable for multiplemolecular biology applications including agarose electrophoresis for the separation of nucleic acids such as DNA and RNA.