Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.0) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Teknova 5M Sodium Chloride Solution. 500 mL, Sterile.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline | MFCD00131855 | 10/pk
Phosphate buffered saline | MFCD00131855 | 10/pk
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 1L
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
STEMCELL Technologies Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum, Size: 500 mL
Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum is recommended for use as a buffer to dilute or wash nucleated cells.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (20X) - 500ml
Phosphate Buffered Saline (PBS) is a buffer solution widely used in biological research due to its isotonic and non-toxic nature. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for applications including protein purification, Western Blotting and immunoassays, sample dilution, and as a transport medium. This buffer is formulated in a 20X concentration, and can be diluted to support its application.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, MFCD00132476, 1 L
Synonym: TBS, 10 ×, solution. Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.
Teknova 10mM Tris-HCl with 0.05% Tween-20, pH 8.0. 250mL, Sterile
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 20X PBS with 1% Tween-20, pH 7.3 (1000mL, Sterile)
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.0) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X) - 4L
The Tris Buffered Saline (TBS) is an isotonic solution frequently used in biological research due to its non-toxic nature. The formulation of TBS includes Tris (tris(hydroxymethyl)aminomethane), which serves as a buffering agent, and Sodium Chloride (NaCl) salt. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerformance Certified, 1 M, suitable for cell culture | MFCD00006181 | 100ML
MOPS solution BioPerformance Certified, 1 M, suitable for cell culture | MFCD00006181 | 100ML