Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (20X) - 500ml
Phosphate Buffered Saline (PBS) is a buffer solution widely used in biological research due to its isotonic and non-toxic nature. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for applications including protein purification, Western Blotting and immunoassays, sample dilution, and as a transport medium. This buffer is formulated in a 20X concentration, and can be diluted to support its application.
Boston Bioproducts Inc MES Buffer (1 M, pH 6.5) - 500ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Mitegen Tris/ HCl, 1M, pH8.5, 100 ml, CAS # 77-86-1
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
TRIBIOSCIENCE INC 2x HBS, pH 7.05 (Sterile, Tissue Culture Grade) 500 ML
Tribioscience’s HBS (2x HEPES buffered saline) is ready to use for chemical transfection, including calcium phosphate transfection and purposes such as calcium transfection, calcium phosphate transfection, mammalian cell transfection, virus production, lentivirus and retrovirus transfection, and AAV transfection.
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 100ML
Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 100ML
Teknova 10mM Tris-HCl with 0.05% Tween-20, pH 8.0. 250mL, Sterile
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Gold Biotechnology Inc Tris (Tris Base) 500 g
GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
Boston Bioproducts Inc Tris Buffered Saline-Tween (TBST), 20X Concentration, 4 x 1 L, 2% Tween-20, pH 7.40 ± 0.20, Wash buffer for immunoassays, Antibody diluent
Tris Buffered Saline-Tween (TBST), 20X Concentration, 4 x 1 L, 2% Tween-20, pH 7.40 ± 0.20, Wash buffer for immunoassays, Antibody diluent
FUJIFILM BIOSCIENCES INC HEPES Buffer Solution 1M
Buffer to maintain pH between 7.2 and 7.6. Suitable for use in cell cultures outside of a CO2 incubator.
STEMCELL Technologies D-PBS (Without Ca++ and Mg++), Size: 500 mL
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating, or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions.
Boston Bioproducts Inc HEPES Buffer, 1 M, pH 7.5, 500 mL
This product is a 1 M HEPES Buffer solution, pH 7.5, which is essential for maintaining stable physiological pH in various biological applications. It is rigorously quality-controlled and specifically formulated for precision in sensitive biochemical and molecular biology research. The high-purity zwitterionic buffering agent is ready-to-use and suitable for diverse applications such as cell culture, protein purification, and live cell imaging.
- Stable pH control maintaining 6.8 to 8.2
- Broad applicability in various research areas
- Convenient format as a ready-to-use solution
- High purity, formulated with USP Compendial-Grade raw materials
- CO₂-independent buffering for reliable pH stability
- Filtered through a 0.22-micron filter for enhanced purity
VWR INTERNATIONAL LLC Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer. Does not contain magnesium or calcium.